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Mercodia Insulin EIA胰岛素检测试剂盒产品介绍：
预期用途:Mercodia Insulin ELISA provides a method for the quantitative determination ofhuman insulin in serum or plasma.
Summary and explanation of the test
Mercodia Insulin EIA胰岛素检测试剂盒Insulin is the principal hormone responsible for the control of glucose metabolism.It is synthesized in the beta-cells of the islets of Langerhans as the precursor,proinsulin,which is processed to form C-peptide and insulin.Both are secretedin equimolar amounts into the portal circulation.The mature insulin moleculecomprises two polypeptide chains,the A chain and B chain(21 and 30 aminoacids respectively).The two chains are linked together by two inter-chaindisulphide bridges.There is also an intra-chain disulphide bridge in the A chain.Secretion of insulin is mainly controlled by plasma glucose concentration,and the hormone has a number of important metabolic actions.Its principalfunction is to control the uptake and utilization of glucose in peripheral tissuesvia the glucose transporter.This and other hypoglycaemic activities,such as theinhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by thehyperglycaemic hormones including glucagon,epinephrine(adrenaline),growthhormone and cortisol.Insulin concentrations are severely reduced in insulin-dependent diabetesmellitus(IDDM)and some other conditions such as hypopituitarism.Insulin levels are raised in non-insulin-dependent diabetes mellitus(NIDDM),obesity,insulinoma and some endocrine dysfunctions such as Cushing’ssyndrome and acromegaly.
Principle of the procedure
Mercodia Insulin ELISA is a solid phase two-site enzyme immunoassay.It isbased on the direct sandwich technique in which two monoclonal antibodies aredirected against separate antigenic determinants on the insulin molecule.During incubation insulin in the sample reacts with peroxidase-conjugatedanti-insulin antibodies and anti-insulin antibodies bound to microplate wells.A simple washing step removes unbound enzyme labelled antibody.The boundconjugate is detected by reaction with 3,3’,5,5’-tetramethylbenzidine(TMB).The reaction is stopped by adding acid to give a colorimetric endpoint that is read。
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